A Phyto-mycotherapeutic Supplement, Namely Ganostile, as Effective Adjuvant in Brain Cancer Management: An In Vitro Study Using U251 Human Glioblastoma Cell Line

Ludovica Gaiaschi, Fabrizio De Luca, Elisa Roda, Beatrice Ferrari, Claudio Casali, Chiara Rita Inguscio, Federica Gola, Enrico Pelloni, Elena Savino, Mauro Ravera, Paola Rossi, Maria Grazia Bottone

Research output: Contribution to journalArticlepeer-review

Abstract

The current standard oncotherapy for glioblastoma is limited by several adverse side effects, leading to a short-term patient survival rate paralleled by a worsening quality of life (QoL). Recently, Complementary and Integrative Medicine’s (CIM) innovative approaches have shown positive impacts in terms of better response to treatment, side effect reduction, and QoL improvement. In particular, promising potential in cancer therapy has been found in compounds coming from phyto- and mycotherapy. The objective of this study was to demonstrate the beneficial effects of a new phyto-mycotherapy supplement, named Ganostile, in the human glioblastoma cell line U251, in combination with chemotherapeutic agents, i.e., Cisplatin and a new platinum-based prodrug. Choosing a supplement dosage that mimicked oral supplementation in humans (about 1 g/day), through in vitro assays, microscopy, and cytometric analysis, it has emerged that the cells, after 48hr continuous exposure to Ganostile in combination with the chemical compounds, showed a higher mortality and a lower proliferation rate than the samples subjected to the different treatments administered individually. In conclusion, our data support the use of Ganostile in integrative oncology protocols as a promising adjuvant able to amplify conventional and new drug effects and also reducing resistance mechanisms often observed in brain tumors.

Original languageEnglish
Article number6204
JournalInternational Journal of Molecular Sciences
Volume25
Issue number11
DOIs
Publication statusPublished - Jun 2024

Keywords

  • drug resistance
  • ferroptosis
  • glioblastoma
  • mitochondrial disfunction
  • oxidative stress
  • phytotherapy

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