A new algorithm for the simulation of SDS 2D-PAGE datasets

Emilio MARENGO; Elisa ROBOTTI

doi:10.1007/978-1-61779-821-4_34

A new algorithm for the simulation of SDS 2D-PAGE datasets

Department of Science and Technological Innovation

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

This chapter describes a new software for the generation of simulated realistic sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis (SDS 2D-PAGE) images. In order to choose the simulation strategy to provide realistic 2D-PAGE maps the statistical characteristics of such images were taken into account, such as the distributions of sizes, intensities, and volumes of the spots. Also, the low reproducibility typical of replicated SDS 2D-PAGE maps of the same sample was simulated. This approach can be used to generate simulated datasets useful in the development and performance evaluation of new classification and/or image analysis algorithms applied to two-dimensional electrophoresis datasets, given the usually small number of experimental replications available.

Original language	English
Title of host publication	"Protein Electrophoresis: methods and protocols"
Publisher	Humana Press, Springer
Pages	407-425
Number of pages	19
Volume	869
ISBN (Print)	9781617798207
DOIs	https://doi.org/10.1007/978-1-61779-821-4_34
Publication status	Published - 2012

Access to Document

10.1007/978-1-61779-821-4_34

http://hdl.handle.net/11579/13832

Cite this

@inbook{8335c0ab35f5453e9614eed7541eec94,

title = "A new algorithm for the simulation of SDS 2D-PAGE datasets",

abstract = "This chapter describes a new software for the generation of simulated realistic sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis (SDS 2D-PAGE) images. In order to choose the simulation strategy to provide realistic 2D-PAGE maps the statistical characteristics of such images were taken into account, such as the distributions of sizes, intensities, and volumes of the spots. Also, the low reproducibility typical of replicated SDS 2D-PAGE maps of the same sample was simulated. This approach can be used to generate simulated datasets useful in the development and performance evaluation of new classification and/or image analysis algorithms applied to two-dimensional electrophoresis datasets, given the usually small number of experimental replications available.",

author = "Emilio MARENGO and Elisa ROBOTTI",

year = "2012",

doi = "10.1007/978-1-61779-821-4\_34",

language = "English",

isbn = "9781617798207",

volume = "869",

pages = "407--425",

booktitle = "{"}Protein Electrophoresis: methods and protocols{"}",

publisher = "Humana Press, Springer",

}

TY - CHAP

T1 - A new algorithm for the simulation of SDS 2D-PAGE datasets

AU - MARENGO, Emilio

AU - ROBOTTI, Elisa

PY - 2012

Y1 - 2012

N2 - This chapter describes a new software for the generation of simulated realistic sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis (SDS 2D-PAGE) images. In order to choose the simulation strategy to provide realistic 2D-PAGE maps the statistical characteristics of such images were taken into account, such as the distributions of sizes, intensities, and volumes of the spots. Also, the low reproducibility typical of replicated SDS 2D-PAGE maps of the same sample was simulated. This approach can be used to generate simulated datasets useful in the development and performance evaluation of new classification and/or image analysis algorithms applied to two-dimensional electrophoresis datasets, given the usually small number of experimental replications available.

AB - This chapter describes a new software for the generation of simulated realistic sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis (SDS 2D-PAGE) images. In order to choose the simulation strategy to provide realistic 2D-PAGE maps the statistical characteristics of such images were taken into account, such as the distributions of sizes, intensities, and volumes of the spots. Also, the low reproducibility typical of replicated SDS 2D-PAGE maps of the same sample was simulated. This approach can be used to generate simulated datasets useful in the development and performance evaluation of new classification and/or image analysis algorithms applied to two-dimensional electrophoresis datasets, given the usually small number of experimental replications available.

UR - https://iris.uniupo.it/handle/11579/13832

U2 - 10.1007/978-1-61779-821-4_34

DO - 10.1007/978-1-61779-821-4_34

M3 - Chapter

SN - 9781617798207

VL - 869

SP - 407

EP - 425

BT - "Protein Electrophoresis: methods and protocols"

PB - Humana Press, Springer

ER -

A new algorithm for the simulation of SDS 2D-PAGE datasets

Abstract

Access to Document

Other files and links

Fingerprint

Cite this